Determining method of tissue cultured seedling sucrose

The invention discloses a determining method of tissue cultured seedling sucrose. The method comprises the following steps of respectively adopting beet sucrose and sugarcane sucrose as organic carbon sources to prepare a beet sucrose culture medium and a sugarcane sucrose culture medium; culturing tissue cultured seedlings with consistent growth vigors until the fresh weight is increased by over 20 times, and finishing the culturing; determining delta C values of the beet sucrose, the sugarcane sucrose and newly-growing leaves; according to the tissue cultured seedlings of plants to be determined, utilizing the sugarcane sucrose and the beet sucrose to produce carbon isotope fractionation values, and utilizing a fractionation value of carbon dioxide in air, the weight increment of the tissue cultured seedlings and sugarcane consumption to calculate the sugarcane utilization rate of the tissue cultured seedlings. The method provided by the invention not only can be used for quantitatively determining the auto