Method for propagating a virus

The present disclosure relates to the field of aquaculture, farmed fish and the occurrence of disease in farmed fish. More particularly, the present disclosure provides means for preparing large amounts of an orthoreovirus, such as piscine reovirus (PRV), by ex vivo propagation in nucleated erythroc...

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Bibliographische Detailangaben
Hauptverfasser: DAHLE MARIA K, FINSTAD OYSTEIN W, RIMSTAD ESPEN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The present disclosure relates to the field of aquaculture, farmed fish and the occurrence of disease in farmed fish. More particularly, the present disclosure provides means for preparing large amounts of an orthoreovirus, such as piscine reovirus (PRV), by ex vivo propagation in nucleated erythrocytes. The present document also provides means for preparing vaccine compositions comprising orthoreovirus. 本公开涉及水产养殖业领域、养殖鱼和在养殖鱼中疾病的发生。更具体地,本公开提供了用于通过在有核红细胞中体外增殖来制备大量诸如鱼呼肠孤病毒(PRV)的正呼肠孤病毒的方法。本文还提供了用于制备包含正呼肠孤病毒的疫苗组合物的方法。