Method for cultivating hepatitis E viruses

The invention discloses a method for cultivating hepatitis E viruses in an in-vitro manner. The method has the advantages that miRNA-A6 [micro-RNA (ribonucleic acid)-A6] is transfected by cells for 24 h, then viruses are inoculated and can be massively replicated, and mass replication of offspring v...

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Bibliographische Detailangaben
Hauptverfasser:	HUANG FEN, WANG JUE, LONG FEIYAN, JING SHENRONG, YANG CHENCHEN, JIANG DIANCAI, BI YANHONG, LI YUNLONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a method for cultivating hepatitis E viruses in an in-vitro manner. The method has the advantages that miRNA-A6 [micro-RNA (ribonucleic acid)-A6] is transfected by cells for 24 h, then viruses are inoculated and can be massively replicated, and mass replication of offspring viruses of the HEV (hepatitis E viruses) can be promoted by the transfected miRNA-A6 as revealed by means of Real-Time qPCR (quantitative polymerase chain reaction) and Western Blot analysis; the method is major breakthrough in the aspect of HEV in-vitro cultivation, and excellent foundation is laid for further researching biological characteristics and immunological characteristics of the HEV, researching and developing HEV vaccine and screening anti-HEV medicines. 本发明公开了一种戊型肝炎病毒在体外培养的方法；该方法通过对细胞转染miRNA-A6？24h后接种病毒，能够使病毒大量复制，利用Real-Time？qPCR和Western？Blot分析揭示了转染miRNA-A6可促进HEV子代病毒大量复制。该发明在HEV体外培养方面是一个大的突破，为进一步研究HEV的生物学特性及免疫学特性，？研发HEV疫苗及抗HEV药物的筛选奠定了良好的基础。