Preparation method of weak acid cation exchange stationary phase

The invention discloses a preparation method of a weak acid cation chromatographic stationary phase with highly cross-linked glycidyl methacrylate-divinyl benzene microspheres as the matrix. The preparation method comprises the following steps: monodispersed linear polystyrene seed microspheres are prepared by a dispersion polymerization method; highly cross-linked glycidyl methacrylate-divinyl benzene copolymer microspheres are prepared by a two-step seed swelling method; and by using succinic anhydride, phthalic anhydride, maleic anhydride and maleic acid as reaction materials, the weak acid cation chromatographic stationary phase is prepared. 本发明公开了一种以高交联甲基丙烯酸缩水甘油酯-二乙烯基苯微球为基质的弱酸阳离子色谱固定相的制备方法，采用分散聚合法制备单分散的线性聚苯乙烯种子微球；采用两步种子溶胀法，制备高交联甲基丙烯酸缩水甘油酯-二乙烯基苯共聚微球；以丁二酸酐，邻苯二甲酸酐，马来酸酐和马来酸为反应原料，制备了一种弱酸型阳离子色谱固定相。