Method for extracting total flavonoids of gnaphalium japonicum

The invention discloses a method for extracting total flavonoids of gnaphalium japonicum. The method comprises the following steps: (1) cleaning gnaphalium japonicum, drying and grinding to obtain gnaphalium japonicum powder; (2) soaking the gnaphalium japonicum powder with ethanol; (3) performing ultrasonic treatment for three times and merging the filtrates, and cooling to obtain extract; (4) vacuumizing the extract, concentrating and removing ethanol to obtain concentrate; (5) performing chromatographic separation of adsorption resin on the concentrate; and after column attachment, washing impurities with purified water and eluting with ethanol to obtain eluate; and (6) performing vacuum concentration of the eluate to obtain yellow extract which is the flavonoids of gnaphalium japonicum. In the method disclosed by the invention, the total flavonoids of gnaphalium japonicum are extracted with ultrasonic for the first time, the method is simple and quick, and the product cost is low; and by adopting macropor