Anti-TOPK antibody with 74th tyrosine residue phosphorylated as well as preparation method and application of anti-TOPK antibody

The invention discloses an anti-TOPK antibody with 74th tyrosine residue phosphorylated as well as a preparation method and an application of anti-TOPK antibody and belongs to the field of biology and disease diagnosis. The antibody is obtained as follows: polypeptides with the amino acid sequence shown in SEQ ID NO: 1 are coupled with carrier protein, a product serves as an antigen, the antigen is used for immunize an animal, polyclonal antibody serums are obtained and purified, and the antibody is obtained. The antibody can be applied to immunoblotting detection of the tumor cell level. Currently, an antibody for detecting Y74 phosphorylation of TOPK is not available in the market. The antibody lays a solid foundation for research of tumor occurrence as well as early diagnosis and prognosis predicted treatment of the tumor. 本发明公开了种抗TOPK第74位酪氨酸残基磷酸化的抗体，属于生物和疾病诊断领域。该抗体是将氨基酸序列如SEQ？ID？NO：1所示的多肽与载体蛋白偶联，用作抗原，并用该抗原免疫动物，得到多抗血清，纯化后得到的。该抗体能应用于肿瘤细胞水平的免疫印迹方法检测，目前市面上尚无针对TOPK？Y74磷酸化检测的任何抗体，本发明为肿瘤发生的研究及肿瘤的早期诊断及预后预测治疗打下了坚实