Method for removing RuBisco protein in broussonetia papyrifera leaf protein

The invention discloses a method for removing RuBisco protein in broussonetia papyrifera leaf protein. The method can be used for removing RuBisco protein in all leaf proteins and comprises the following steps: (1) preparing homogenate; (2) centrifuging the homogenate; (3) centrifuging first supernate; (4) centrifuging second supernate; and (5) washing coarse protein precipitate. The method can be used for extracting leaf proteins of plants, reducing interference of high-abundant protein RuBisco, improving 2-DE electrophoresis resolution ratio and laying the foundation for optimizing detection of the leaf proteins of the plants and further improving varieties by using biotechnological manners. 本发明公开了种去除构树叶片蛋白质中RuBisco蛋白的方法。本发明所提供的方法可用于去除所有叶片蛋白质中RuBisco蛋白，包括如下步骤：(1)制备匀浆；(2)匀浆离心；(3)第上清液离心；(4)第二上清液离心；(5)蛋白粗沉淀的洗涤。用本发明的方法提取植物叶片蛋白，有利于减少高丰度蛋白RuBisco干扰、提高2-DE电泳分辨率，为优化植物叶片蛋白质组检测，运用生物技术手段进步改良品种打下基础。