Porcine circovirus inactivated vaccine efficacy testing method

Porcine circovirus inactivated vaccine efficacy testing method

The invention discloses a porcine circovirus inactivated vaccine efficacy testing method which includes immunizing an experimental animal once or twice with porcine circovirus inactivated vaccine, collecting animal serum sterilely, subjecting the sterilely collected animal serum to doubling dilution...

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Hauptverfasser: CHEN SHENGSHENG, ZHU HONGJIE, CHEN JINGRONG, LIN GONGYANG, WU JINFEI, WU RUNSHENG, ZHAN XIANQIANG, SU GUOHUA, ZHU ZHIGANG
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Sprache:chi ; eng
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INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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creator CHEN SHENGSHENG
ZHU HONGJIE
CHEN JINGRONG
LIN GONGYANG
WU JINFEI
WU RUNSHENG
ZHAN XIANQIANG
SU GUOHUA
ZHU ZHIGANG
description The invention discloses a porcine circovirus inactivated vaccine efficacy testing method which includes immunizing an experimental animal once or twice with porcine circovirus inactivated vaccine, collecting animal serum sterilely, subjecting the sterilely collected animal serum to doubling dilution with maintenance media after inactivation, neutralizing the diluted solution with porcine circovirus antigen containing 200TCID /0.1mL at the environment of 37 DEG C for 30 to 60 minutes, inoculating a neutralization solution into a 96-pore cell plate which is used for growth of cells PK-15, placing the cell plate in a CO2 incubator at 37 DEG C to culture, staining to observe by means of indirect immunofluorescence, calculating neutralizing titer of serum antibody and determining porcine circovirus inactivated vaccine titer. By the method, serum neutralizing antibody titer, generated from porcine circovirus inactivated vaccine, to the experimental animal after the experimental animal is immunized can be tested qua
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Porcine circovirus inactivated vaccine efficacy testing method
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