Porcine circovirus inactivated vaccine efficacy testing method

The invention discloses a porcine circovirus inactivated vaccine efficacy testing method which includes immunizing an experimental animal once or twice with porcine circovirus inactivated vaccine, collecting animal serum sterilely, subjecting the sterilely collected animal serum to doubling dilution with maintenance media after inactivation, neutralizing the diluted solution with porcine circovirus antigen containing 200TCID /0.1mL at the environment of 37 DEG C for 30 to 60 minutes, inoculating a neutralization solution into a 96-pore cell plate which is used for growth of cells PK-15, placing the cell plate in a CO2 incubator at 37 DEG C to culture, staining to observe by means of indirect immunofluorescence, calculating neutralizing titer of serum antibody and determining porcine circovirus inactivated vaccine titer. By the method, serum neutralizing antibody titer, generated from porcine circovirus inactivated vaccine, to the experimental animal after the experimental animal is immunized can be tested qua