HPLC (High Performance Liquid Chromatography) analysis method for sodium 3,3'-dithiodipropane sulfonate

The invention discloses a HPLC (High Performance Liquid Chromatography) analysis method for sodium 3,3'-dithiodipropane sulfonate and belongs to the technical field of analysis. The HPLC analysis method is characterized in that a high performance liquid chromatograph with an ultraviolet detecto...

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Bibliographische Detailangaben
Hauptverfasser: MING RUITAO, ZHOU SHIJUN, YANG WEI, HU ZHE, HUANG KAIWEI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a HPLC (High Performance Liquid Chromatography) analysis method for sodium 3,3'-dithiodipropane sulfonate and belongs to the technical field of analysis. The HPLC analysis method is characterized in that a high performance liquid chromatograph with an ultraviolet detector is used for determining; octadecyl silane is taken as a filling column, the size of a chromatographic column is 250mm (length)*4.6mm (inner diameter) and the grain size is 5 microns; methyl alcohol and a buffer solution in volume ratio of (0-50):(100-50) is taken as a flowing phase; the detection wavelength is 190-330nm, and the column temperature is at 10-60 DEG C; the speed of the flowing phase is 0.3-1.2mL/min; the feeding size of a sample is 20 micro liter; the buffer solution contains 0.17mol/L acetic acid, 0.142mol/L triethylamine and ultrapure water. The content of sodium 3,3'-dithiodipropane sulfonate in a to-be-detected sample can be quickly and accurately measured according to a standard curve established vi