Measuring method for activity of glucose oxidase

The invention relates to a measuring method for the activity of glucose oxidase. The measuring method specifically includes the steps that 1, phosphate buffer with the pH value of 5.8 is prepared, and then a horse radish peroxidase solution, a glucose oxidase solution, guaiacol and a glucose solution are sequentially added at room temperature; 2, an ultraviolet-visible spectrophotometer is adopted for time scanning, and change data of light absorption values of a product tetra-methoxy phenol along with time is obtained; 3, the light absorption values of tetra-methoxy phenol are converted into concentration according to the lambert-beer law, time serves as horizontal coordinates, concentration serves as longitudinal coordinates, a scatter diagram is drawn in excel, a trend line is added, the slope of the trend line is the generation rate of tetra-methoxy phenol, the reaction rate of glucose oxidase catalyzed glucose is obtained by multiplying the generation rate by the coefficient 4, and the activity of the gl