Method for performing DNA terminal repair/dA addition by adopting one-step method and application

The invention belongs to the technical field of molecular biology and particularly relates to a method for performing DNA terminal repair/dA addition by adopting a one-step method and application thereof. The method adopts an enzyme system of T4DNA polymerase, Taq DNA polymerase and T4 polynucleotid...

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Bibliographische Detailangaben
Hauptverfasser:	ZHU YUEYAN, SUN ZIKUI, LI CHANGE, WANG GUO, WU SHUAILAI, WANG FENG, DING FANGMEI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the technical field of molecular biology and particularly relates to a method for performing DNA terminal repair/dA addition by adopting a one-step method and application thereof. The method adopts an enzyme system of T4DNA polymerase, Taq DNA polymerase and T4 polynucleotide kinase. The time required by the DNA terminal repair/dA addition in the method is shortened from one and a half hours to 40 minutes, and the reagent consumable consumption is reduced due to the fact that a DNA purification step is omitted, the cost is saved, DNA loss caused by purification is avoided, the preparation efficiency of a DNA library is improved, and the lowest DNA quality required by preparation of the DNA library is reduced.