Agar gel precipitin antigen of duck hepatitis virus, preparation method for agar gel precipitin antigen and application of agar gel precipitin antigen

The invention provides an agar gel precipitin antigen of a duck hepatitis virus, a preparation method for the agar gel precipitin antigen and application of the agar gel precipitin antigen. The agar gel precipitin antigen of the duck hepatitis virus is obtained in a manner of inoculating a large num...

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Hauptverfasser: CUI ZHIGANG, ZHEN PANPAN, SONG XIUMEI, JIAO XIAOJUN, LI LIQIN, YANG XUE, ZHU SHIJIANG, WANG YONG, WANG MENG, YU XIAOTING, WANG JIAN, JIANG LINJIE, ZHANG LIHUI, CHENG XUEJIAO, GE ZHAONING, WANG YUPENG, YU GUIJU
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides an agar gel precipitin antigen of a duck hepatitis virus, a preparation method for the agar gel precipitin antigen and application of the agar gel precipitin antigen. The agar gel precipitin antigen of the duck hepatitis virus is obtained in a manner of inoculating a large number of shelduck embryos to the duck hepatitis virus for cultivation and proliferation, collecting embryo allantoic liquid, concentrating the virus through low-temperature ultracentrifugation, and purifying the virus through sucrose gradient centrifugation and ultracentrifugation to obtain the agar gel precipitin antigen of the duck hepatitis virus; by optimizing conditions, optimal reaction concentration between the antigen and an antibody is determined; proved by a specificity test, a repeatability test and a sensitivity test, the method is simple and feasible. As a detection method for a duck hepatitis virus antibody, established by the agar gel precipitin antigen provided by the invention, has the characteristic