Cryopreservation liquid for horse semen and preparation method of cryopreservation liquid

The invention discloses a cryopreservation liquid for horse semen. 100 mL of the cryopreservation liquid for horse semen is prepared from 2.00-2.40 g of glucose, 3.40-4.00 g of lactose, 0.60-1.00 g of trehalose, 0.061-0.092 g of glutathione, 0.50-0.70 g of sodium citrate, 30-50 mL of skimmed milk, 2.5-4.5 mL of egg yolk, 50,000-100,000 IU of penicillin, 50,000-100,000 IU of streptomycin, 3.5-5.5 mL of glycerin and double distilled water. The invention further discloses a preparation method of the cryopreservation liquid. The cryopreservation liquid for horse semen can effectively increase the survival rate and semen activity of the horse semen during cryopreservation. The unfrozen semen has high physiological indexes, has the stable and reliable fecundation effect after field application and can be applied to production. The preparation method comprises few steps and is simple and easy to operate, and the preparation condition is mild and easy to control.