Multiplex PCR (polymerase chain reaction) detection method of tobacco Ty-2 gene and Ty-5 gene
The invention discloses a multiplex PCR (polymerase chain reaction) detection method of tobacco Ty-2 gene and Ty-5 gene. The detection method comprises the following steps: 1) extracting to-be-detected tobacco leaf DNA from tobacco leaves; 2) by taking the DNA extracted from the tobacco leaves as a...
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| creator | YU WENGUI YANG MALI LI YARU WANG YINLEI ZHAO LIPING ZHAO TONGMIN JIANG JING |
| description | The invention discloses a multiplex PCR (polymerase chain reaction) detection method of tobacco Ty-2 gene and Ty-5 gene. The detection method comprises the following steps: 1) extracting to-be-detected tobacco leaf DNA from tobacco leaves; 2) by taking the DNA extracted from the tobacco leaves as a template, conducting multiplex PCR amplification on a to-be-detected sample by an established multiplex PCR reaction system by virtue of primers of Ty-3 and Ty-5; 3) conducting electrophoresis detection on a PCR product; 4) identifying whether the to-be-detected tobacco sample contains the Ty-2 gene and Ty-5 gene or not; and the like. The detection method disclosed by the invention is used for rapidly identifying whether tobacco yellow leaf curl resistant genes Ty-2 gene and Ty-5 exist in a tobacco material or not; therefore, the detection method is beneficial for screening disease-resistant gene materials when pyramiding breeding is conducted on the two genes. |
| format | Patent |
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The detection method comprises the following steps: 1) extracting to-be-detected tobacco leaf DNA from tobacco leaves; 2) by taking the DNA extracted from the tobacco leaves as a template, conducting multiplex PCR amplification on a to-be-detected sample by an established multiplex PCR reaction system by virtue of primers of Ty-3 and Ty-5; 3) conducting electrophoresis detection on a PCR product; 4) identifying whether the to-be-detected tobacco sample contains the Ty-2 gene and Ty-5 gene or not; and the like. The detection method disclosed by the invention is used for rapidly identifying whether tobacco yellow leaf curl resistant genes Ty-2 gene and Ty-5 exist in a tobacco material or not; therefore, the detection method is beneficial for screening disease-resistant gene materials when pyramiding breeding is conducted on the two genes.</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160406&DB=EPODOC&CC=CN&NR=105463080A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25555,76308</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160406&DB=EPODOC&CC=CN&NR=105463080A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>YU WENGUI</creatorcontrib><creatorcontrib>YANG MALI</creatorcontrib><creatorcontrib>LI YARU</creatorcontrib><creatorcontrib>WANG YINLEI</creatorcontrib><creatorcontrib>ZHAO LIPING</creatorcontrib><creatorcontrib>ZHAO TONGMIN</creatorcontrib><creatorcontrib>JIANG JING</creatorcontrib><title>Multiplex PCR (polymerase chain reaction) detection method of tobacco Ty-2 gene and Ty-5 gene</title><description>The invention discloses a multiplex PCR (polymerase chain reaction) detection method of tobacco Ty-2 gene and Ty-5 gene. The detection method comprises the following steps: 1) extracting to-be-detected tobacco leaf DNA from tobacco leaves; 2) by taking the DNA extracted from the tobacco leaves as a template, conducting multiplex PCR amplification on a to-be-detected sample by an established multiplex PCR reaction system by virtue of primers of Ty-3 and Ty-5; 3) conducting electrophoresis detection on a PCR product; 4) identifying whether the to-be-detected tobacco sample contains the Ty-2 gene and Ty-5 gene or not; and the like. The detection method disclosed by the invention is used for rapidly identifying whether tobacco yellow leaf curl resistant genes Ty-2 gene and Ty-5 exist in a tobacco material or not; therefore, the detection method is beneficial for screening disease-resistant gene materials when pyramiding breeding is conducted on the two genes.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2016</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNirEKwjAUALs4iPoPz02HQrRWXCUoLopIVymvyasNpHmhiWD_Xi1-gNPdwY2T-_lpo_GWXnCVN1h4tn1LHQYC1aBx0BGqaNgtQVOkQaGl2LAGriFyhUoxFH26hgc5AnT6W_lQ02RUow00-3GSzI-HQp5S8lxS8Kg-UyzlZSXyzTYTO7HP_nneFK06KQ</recordid><startdate>20160406</startdate><enddate>20160406</enddate><creator>YU WENGUI</creator><creator>YANG MALI</creator><creator>LI YARU</creator><creator>WANG YINLEI</creator><creator>ZHAO LIPING</creator><creator>ZHAO TONGMIN</creator><creator>JIANG JING</creator><scope>EVB</scope></search><sort><creationdate>20160406</creationdate><title>Multiplex PCR (polymerase chain reaction) detection method of tobacco Ty-2 gene and Ty-5 gene</title><author>YU WENGUI ; YANG MALI ; LI YARU ; WANG YINLEI ; ZHAO LIPING ; ZHAO TONGMIN ; JIANG JING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN105463080A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2016</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>YU WENGUI</creatorcontrib><creatorcontrib>YANG MALI</creatorcontrib><creatorcontrib>LI YARU</creatorcontrib><creatorcontrib>WANG YINLEI</creatorcontrib><creatorcontrib>ZHAO LIPING</creatorcontrib><creatorcontrib>ZHAO TONGMIN</creatorcontrib><creatorcontrib>JIANG JING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YU WENGUI</au><au>YANG MALI</au><au>LI YARU</au><au>WANG YINLEI</au><au>ZHAO LIPING</au><au>ZHAO TONGMIN</au><au>JIANG JING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Multiplex PCR (polymerase chain reaction) detection method of tobacco Ty-2 gene and Ty-5 gene</title><date>2016-04-06</date><risdate>2016</risdate><abstract>The invention discloses a multiplex PCR (polymerase chain reaction) detection method of tobacco Ty-2 gene and Ty-5 gene. The detection method comprises the following steps: 1) extracting to-be-detected tobacco leaf DNA from tobacco leaves; 2) by taking the DNA extracted from the tobacco leaves as a template, conducting multiplex PCR amplification on a to-be-detected sample by an established multiplex PCR reaction system by virtue of primers of Ty-3 and Ty-5; 3) conducting electrophoresis detection on a PCR product; 4) identifying whether the to-be-detected tobacco sample contains the Ty-2 gene and Ty-5 gene or not; and the like. The detection method disclosed by the invention is used for rapidly identifying whether tobacco yellow leaf curl resistant genes Ty-2 gene and Ty-5 exist in a tobacco material or not; therefore, the detection method is beneficial for screening disease-resistant gene materials when pyramiding breeding is conducted on the two genes.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Multiplex PCR (polymerase chain reaction) detection method of tobacco Ty-2 gene and Ty-5 gene |
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