Preparation method of micro-and nanoscale protein crystals capable of stably existing in air

Preparation method of micro-and nanoscale protein crystals capable of stably existing in air

The invention provides a preparation method of micro-and nanoscale protein crystals capable of stably existing in air. Protein mini-crystals grow through the simple and feasible method, chemical cross-linking is conducted on the mini-crystals through a cross-linking agent, and the protein mini-cryst...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: YAN ERKAI, CHEN DA, LIU YUE, LIU YALI, YIN DACHUAN, ZHOU RENBIN, ZHANG CHENYAN, LI DAWEI
Format: Patent
Sprache:chi ; eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention provides a preparation method of micro-and nanoscale protein crystals capable of stably existing in air. Protein mini-crystals grow through the simple and feasible method, chemical cross-linking is conducted on the mini-crystals through a cross-linking agent, and the protein mini-crystals capable of stably existing in air are obtained. The method is convenient to operate, economical and practical; the prepared protein mini-crystals do not need crystal rings or capillary tubes when used for free electron laser diffraction, and background scattering can be eliminated; when the crystals are used as a catalyst, a sample can not be dissolved and can be repeatedly used.