A-type H5N6 subtype avian influenza virus dual-channel real-time fluorescence PCR (polymerase chain reaction) detection kit and detection method

The invention discloses a detection kit for detecting A-type H5N6 subtype avian influenza virus by utilizing dual fluorescence PCR (polymerase chain reaction). The detection kit comprises a PCR primer for amplifying H5 and N6 genes and a probe, the nucleotide sequence is shown in Seq ID No: 1-6, a fluorescence reporter group is marked at the 5' terminal of the probe, and a fluorescence quenching group is marked at 3 terminal; a reaction system comprises an RT-PCR reaction liquid, an enzyme mixing liquid, a positive standard substance and a negative standard substance. The invention further discloses a method for detection by utilizing the kit. The method comprises steps such as extraction of sample nucleic acid, preparation of a reaction system, amplification through fluorogenic quantitative PCR, result reading and the like. The detection kit can rapidly detect the A-type H5N6 subtype avian influenza virus and has the advantages of simplicity in operation, high sensitivity, good specificity and the like, and