Panaxnotoginseng transcription factor gene PnbHLH1 and application thereof

The invention discloses a panaxnotoginseng transcription factor gene PnbHLH1 and application thereof, i.e. the application in increasing the expression quantity of key enzyme genes during biosynthesis of saponins of panaxnotoginseng and increasing the content of total saponins and a monomer saponin...

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Bibliographische Detailangaben
Hauptverfasser:	HUANG ZHUANGJIA, LIU DIQIU, GE FENG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a panaxnotoginseng transcription factor gene PnbHLH1 and application thereof, i.e. the application in increasing the expression quantity of key enzyme genes during biosynthesis of saponins of panaxnotoginseng and increasing the content of total saponins and a monomer saponin in panaxnotoginseng callus. The panax notoginseng transcription factor gene PnWRKY1 has a nucleotide sequence shown as SEQ ID NO: 1, and codes a WRKY type transcription factor. According to the panaxnotoginseng transcription factor gene PnbHLH1, functional genomics and metabolic engineering related techniques verify that the panaxnotoginseng PnbHLH1 transcription factor has the function of positively regulating the biosynthesis of the saponins of panaxnotoginseng; when the panaxnotoginseng PnbHLH1 transcription factor gene is constructed to a plant expression vector and is transferred into the panaxnotoginseng callus for overexpression, the expression quantity of the key enzyme genes in a synthetic pathway of the s