Detection kit and method for isothermal amplification of nucleic acid of Shigella
The invention discloses a detection kit and method for isothermal amplification of nucleic acid of Shigella. The detection kit comprises a DNA extraction reagent, isothermal amplification reaction fluid, positive control and negative control. The detection kit is high in specificity and senstitivity...
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| creator | ZHAN LI MEI LINGLING XU CHANGPING LU YIYU XU ZHOUHENG FENG YAN |
| description | The invention discloses a detection kit and method for isothermal amplification of nucleic acid of Shigella. The detection kit comprises a DNA extraction reagent, isothermal amplification reaction fluid, positive control and negative control. The detection kit is high in specificity and senstitivity; the whole nucleic acid amplification reaction is kept at the same constant temperature, isothermal amplification only needs 35 min, a detection result of a nucleic acid test strip needs 1-2 min, the whole detection process only needs about 1h from receiving a sample to obtaining the result, and only one thermostat is needed in the whole reaction process, so that the detection kit is particularly suitable for quick detection and elimination on site of Shigella. |
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The detection kit comprises a DNA extraction reagent, isothermal amplification reaction fluid, positive control and negative control. The detection kit is high in specificity and senstitivity; the whole nucleic acid amplification reaction is kept at the same constant temperature, isothermal amplification only needs 35 min, a detection result of a nucleic acid test strip needs 1-2 min, the whole detection process only needs about 1h from receiving a sample to obtaining the result, and only one thermostat is needed in the whole reaction process, so that the detection kit is particularly suitable for quick detection and elimination on site of Shigella.</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160323&DB=EPODOC&CC=CN&NR=105420355A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160323&DB=EPODOC&CC=CN&NR=105420355A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHAN LI</creatorcontrib><creatorcontrib>MEI LINGLING</creatorcontrib><creatorcontrib>XU CHANGPING</creatorcontrib><creatorcontrib>LU YIYU</creatorcontrib><creatorcontrib>XU ZHOUHENG</creatorcontrib><creatorcontrib>FENG YAN</creatorcontrib><title>Detection kit and method for isothermal amplification of nucleic acid of Shigella</title><description>The invention discloses a detection kit and method for isothermal amplification of nucleic acid of Shigella. The detection kit comprises a DNA extraction reagent, isothermal amplification reaction fluid, positive control and negative control. 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The detection kit comprises a DNA extraction reagent, isothermal amplification reaction fluid, positive control and negative control. The detection kit is high in specificity and senstitivity; the whole nucleic acid amplification reaction is kept at the same constant temperature, isothermal amplification only needs 35 min, a detection result of a nucleic acid test strip needs 1-2 min, the whole detection process only needs about 1h from receiving a sample to obtaining the result, and only one thermostat is needed in the whole reaction process, so that the detection kit is particularly suitable for quick detection and elimination on site of Shigella.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Detection kit and method for isothermal amplification of nucleic acid of Shigella |
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