Identification method for conventional rice varieties Huai rice No.5 and No.18 based on InDel marks
The invention belongs to the technical field of creature recognition and particularly provides an identification method for conventional rice varieties Huai rice No.5 and No.18 based on InDel marks. 24 pairs of peculiar DNA primers designed according to inserted/missing difference segments obtained...
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Zusammenfassung: | The invention belongs to the technical field of creature recognition and particularly provides an identification method for conventional rice varieties Huai rice No.5 and No.18 based on InDel marks. 24 pairs of peculiar DNA primers designed according to inserted/missing difference segments obtained through comparison of whole-genome DNA sequences of a japonica rice variety Nipponbare and an indica type rice variety 93-11 are used for performing DNA extraction, DNA segment amplification, electrophoretic separation and electrophoretogram analysis on Huai rice No.5 and No.18 to identify the Huai rice No.5 and No.18 varieties. Specifically, 24 pairs of InDel primers are used for analyzing a fingerprint spectrum obtained on the basis of a polymerase chain reaction and vertical gel plate electrophoresis, and then whether the varieties are conventional rice varieties Huai rice No.5 and No.18 or not is determined according to the electrophoretic band type of 24 InDel loci. The number of required detected samples is s |
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