Method for preparing oat beta-glucan through ball milling

The invention relates to a method for preparing oat beta-glucan through ball milling, which belongs to the technical field of deep processing of oat. The method comprises the following steps: (1) pretreatment; (2) degreasing treatment; (3) microwave drying; (4) extraction with alkaline water; (5) centrifugation of filtrate; (6) ultrasonic treatment; (7) treatment with amylase; (8) treatment with glucoamylase; (9) centrifugation of enzymatic hydrolysis liquid; (10) isoelectric precipitation; (11) centrifugation after enzyme deactivation; (12) concentration and precipitation; (13) centrifugation and collection of precipitate; and (14) freeze drying. The method provided by the invention employs a combination of mechanochemical technology (ball milling) and enzymatic hydrolysis technology to prepare oat beta-glucan and fills a blank in a mechanochemical process for preparation of oat beta-glucan; and through ball milling, enzymatic hydrolysis time is shortened, enzymatic hydrolysis effect is improved, and the val