Kit capable of being used for detecting SHISA3 gene promotor region methylation level related to laryngocarcinoma and application thereof

The invention discloses a kit capable of being used for detecting SHISA3 gene promotor region methylation level related to laryngocarcinoma and application thereof. The kit is characterized in that the kit comprises a pair of SHISA3 gene promotor region methylation specific amplification primers and a pair of non-methylation specific amplification primers, wherein a nucleotide sequence of a methylation specific sense primer is shown as SEQ ID NO. 1, a nucleotide sequence of a reverse primer is shown as SEQ ID NO. 2, a nucleotide sequence of a non-methylation specific sense primer is shown as SEQ ID NO. 3, and a nucleotide sequence of a reverse primer is shown as SEQ ID NO. 4. The kit has the advantages that the diagnostic kit can be used for conveniently and quickly realizing detection on laryngocarcinoma at a molecular level, is high in detection efficiency and strong in pertinence; meanwhile, a drug taking SHISA3 gene promotor region methylation as a target spot is expected to become a new means for auxilia