PPV (porcine parvovirus) LAMP (loop-mediated isothermal amplification) kit and application thereof
The invention discloses a PPV (porcine parvovirus) loop-mediated isothermal amplification kit and application thereof. The kit comprises LAMP primers, a 2*reaction buffer solution, Bst DNA polymerase, a fluorescent visual detection reagent, ultrapure water and a PPV DNA template, wherein the LAMP pr...
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| creator | QIN YIBIN HE YING SU QIANLIAN LIANG JIAXING CHEN ZHONGWEI ZHOU YINGNING YANG SIYI DUAN QUNPENG LI BIN JIANG DONGFU ZHAO WU LU BINGXIA |
| description | The invention discloses a PPV (porcine parvovirus) loop-mediated isothermal amplification kit and application thereof. The kit comprises LAMP primers, a 2*reaction buffer solution, Bst DNA polymerase, a fluorescent visual detection reagent, ultrapure water and a PPV DNA template, wherein the LAMP primers comprise outer primers F3 and B3, inner primers FIP and BIP, and loop primers LF and LB. The application lies in that the kit is used for detecting PPV lesion tissue samples and carrying out qualitative research on PPV pathogens. Detection and sensitivity detection results prove that the LAMP detection method provided by the invention can monitor the reaction in real time, and quantitatively detect the copy number of PPV, quickly and accurately obtains the detection result, and brings convenience to easy, convenient, quick and reliable PPV detection. |
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The kit comprises LAMP primers, a 2*reaction buffer solution, Bst DNA polymerase, a fluorescent visual detection reagent, ultrapure water and a PPV DNA template, wherein the LAMP primers comprise outer primers F3 and B3, inner primers FIP and BIP, and loop primers LF and LB. The application lies in that the kit is used for detecting PPV lesion tissue samples and carrying out qualitative research on PPV pathogens. Detection and sensitivity detection results prove that the LAMP detection method provided by the invention can monitor the reaction in real time, and quantitatively detect the copy number of PPV, quickly and accurately obtains the detection result, and brings convenience to easy, convenient, quick and reliable PPV detection.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160224&DB=EPODOC&CC=CN&NR=105349702A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160224&DB=EPODOC&CC=CN&NR=105349702A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>QIN YIBIN</creatorcontrib><creatorcontrib>HE YING</creatorcontrib><creatorcontrib>SU QIANLIAN</creatorcontrib><creatorcontrib>LIANG JIAXING</creatorcontrib><creatorcontrib>CHEN ZHONGWEI</creatorcontrib><creatorcontrib>ZHOU YINGNING</creatorcontrib><creatorcontrib>YANG SIYI</creatorcontrib><creatorcontrib>DUAN QUNPENG</creatorcontrib><creatorcontrib>LI BIN</creatorcontrib><creatorcontrib>JIANG DONGFU</creatorcontrib><creatorcontrib>ZHAO WU</creatorcontrib><creatorcontrib>LU BINGXIA</creatorcontrib><title>PPV (porcine parvovirus) LAMP (loop-mediated isothermal amplification) kit and application thereof</title><description>The invention discloses a PPV (porcine parvovirus) loop-mediated isothermal amplification kit and application thereof. 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The kit comprises LAMP primers, a 2*reaction buffer solution, Bst DNA polymerase, a fluorescent visual detection reagent, ultrapure water and a PPV DNA template, wherein the LAMP primers comprise outer primers F3 and B3, inner primers FIP and BIP, and loop primers LF and LB. The application lies in that the kit is used for detecting PPV lesion tissue samples and carrying out qualitative research on PPV pathogens. Detection and sensitivity detection results prove that the LAMP detection method provided by the invention can monitor the reaction in real time, and quantitatively detect the copy number of PPV, quickly and accurately obtains the detection result, and brings convenience to easy, convenient, quick and reliable PPV detection.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | PPV (porcine parvovirus) LAMP (loop-mediated isothermal amplification) kit and application thereof |
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