PPV (porcine parvovirus) LAMP (loop-mediated isothermal amplification) kit and application thereof

PPV (porcine parvovirus) LAMP (loop-mediated isothermal amplification) kit and application thereof

The invention discloses a PPV (porcine parvovirus) loop-mediated isothermal amplification kit and application thereof. The kit comprises LAMP primers, a 2*reaction buffer solution, Bst DNA polymerase, a fluorescent visual detection reagent, ultrapure water and a PPV DNA template, wherein the LAMP pr...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: QIN YIBIN, HE YING, SU QIANLIAN, LIANG JIAXING, CHEN ZHONGWEI, ZHOU YINGNING, YANG SIYI, DUAN QUNPENG, LI BIN, JIANG DONGFU, ZHAO WU, LU BINGXIA
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROCESSES USING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention discloses a PPV (porcine parvovirus) loop-mediated isothermal amplification kit and application thereof. The kit comprises LAMP primers, a 2*reaction buffer solution, Bst DNA polymerase, a fluorescent visual detection reagent, ultrapure water and a PPV DNA template, wherein the LAMP primers comprise outer primers F3 and B3, inner primers FIP and BIP, and loop primers LF and LB. The application lies in that the kit is used for detecting PPV lesion tissue samples and carrying out qualitative research on PPV pathogens. Detection and sensitivity detection results prove that the LAMP detection method provided by the invention can monitor the reaction in real time, and quantitatively detect the copy number of PPV, quickly and accurately obtains the detection result, and brings convenience to easy, convenient, quick and reliable PPV detection.