C-KIT gene D816V mutation fluorescent PCR detection kit
The invention provides a C-KIT gene D816V mutation fluorescent PCR detection kit which comprises a D816V mutation detection reaction liquid. The D816V mutation detection reaction liquid comprises a 10*PCR buffer solution, deoxyribonucleoside triphosphate, a forward primer and a reverse primer which...
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| creator | DAI LIZHONG GAO TANGJIE WU KANG LONG FENGYING |
| description | The invention provides a C-KIT gene D816V mutation fluorescent PCR detection kit which comprises a D816V mutation detection reaction liquid. The D816V mutation detection reaction liquid comprises a 10*PCR buffer solution, deoxyribonucleoside triphosphate, a forward primer and a reverse primer which are used for amplification of target polynucleotide, and a probe used for detection of target polynucleotide. The C-KIT gene D816V mutation fluorescent PCR detection kit provided by the invention is a detection kit based on a real-time fluorescent quantitative PCR technology and an ARMS-PCR technology, is very high in specificity, quick to operate, simple and convenient in method, and high in detection precision, and can effectively prevent false-negative results due to an internal standard added in a reaction system. |
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The D816V mutation detection reaction liquid comprises a 10*PCR buffer solution, deoxyribonucleoside triphosphate, a forward primer and a reverse primer which are used for amplification of target polynucleotide, and a probe used for detection of target polynucleotide. The C-KIT gene D816V mutation fluorescent PCR detection kit provided by the invention is a detection kit based on a real-time fluorescent quantitative PCR technology and an ARMS-PCR technology, is very high in specificity, quick to operate, simple and convenient in method, and high in detection precision, and can effectively prevent false-negative results due to an internal standard added in a reaction system.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160224&DB=EPODOC&CC=CN&NR=105349682A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160224&DB=EPODOC&CC=CN&NR=105349682A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>DAI LIZHONG</creatorcontrib><creatorcontrib>GAO TANGJIE</creatorcontrib><creatorcontrib>WU KANG</creatorcontrib><creatorcontrib>LONG FENGYING</creatorcontrib><title>C-KIT gene D816V mutation fluorescent PCR detection kit</title><description>The invention provides a C-KIT gene D816V mutation fluorescent PCR detection kit which comprises a D816V mutation detection reaction liquid. 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The D816V mutation detection reaction liquid comprises a 10*PCR buffer solution, deoxyribonucleoside triphosphate, a forward primer and a reverse primer which are used for amplification of target polynucleotide, and a probe used for detection of target polynucleotide. The C-KIT gene D816V mutation fluorescent PCR detection kit provided by the invention is a detection kit based on a real-time fluorescent quantitative PCR technology and an ARMS-PCR technology, is very high in specificity, quick to operate, simple and convenient in method, and high in detection precision, and can effectively prevent false-negative results due to an internal standard added in a reaction system.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | C-KIT gene D816V mutation fluorescent PCR detection kit |
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