New type nucleic acid detection method and detector tube
The invention discloses a new type nucleic acid detection method. Lysate, cleanout fluid and reaction liquid in a detector tube are isolated by arranging a plurality of separating layers in the detector tube, meanwhile, through the control on an external heat source, the separating layers are respec...
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| creator | HU LIN JIN RONGYU FAN YE GAO QIUPING WANG DAISANG ZHANG JIANXUN QI CHEN WANG YU ZHAO XIN SUN GANG PENG SHANMING YU ZHUJUN YU JUNWEI YOU QIMIN FU JING ZHOU YANQIONG WANG SHA ZHU LUOLUO WANG CHEN FAN XINLONG WU ZHIHONG ZHANG QIANGZHONG HUANG LONGMEI HE JUNLI |
| description | The invention discloses a new type nucleic acid detection method. Lysate, cleanout fluid and reaction liquid in a detector tube are isolated by arranging a plurality of separating layers in the detector tube, meanwhile, through the control on an external heat source, the separating layers are respectively melted in different detection stages so as to control time sequence in all detection stages, a mixing device which is controlled by an external magnetic body to move and can be magnetized is used in the lysate, mixes samples added into the detector tube, and nucleic acid extraction and purification of the samples are realized, then nano magnetic beads in the detector tube are driven by the external magnetic body to pass the cleanout fluid and then enter into the reaction liquid, nucleic acid carried by the nano magnetic beads is purified in the cleanout fluid and then is eluted in the reaction liquid, an amplified reaction of the nucleic acid in the reaction liquid occurs, finally, an external device realizes the detection on the sample nucleic acid by the optical detection, so that a plurality of steps of performing extraction, purification, cleaning, elution and amplified reaction on the nucleic acid in one detector tube are realized. |
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Lysate, cleanout fluid and reaction liquid in a detector tube are isolated by arranging a plurality of separating layers in the detector tube, meanwhile, through the control on an external heat source, the separating layers are respectively melted in different detection stages so as to control time sequence in all detection stages, a mixing device which is controlled by an external magnetic body to move and can be magnetized is used in the lysate, mixes samples added into the detector tube, and nucleic acid extraction and purification of the samples are realized, then nano magnetic beads in the detector tube are driven by the external magnetic body to pass the cleanout fluid and then enter into the reaction liquid, nucleic acid carried by the nano magnetic beads is purified in the cleanout fluid and then is eluted in the reaction liquid, an amplified reaction of the nucleic acid in the reaction liquid occurs, finally, an external device realizes the detection on the sample nucleic acid by the optical detection, so that a plurality of steps of performing extraction, purification, cleaning, elution and amplified reaction on the nucleic acid in one detector tube are realized.</description><language>eng</language><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160224&DB=EPODOC&CC=CN&NR=105349530A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160224&DB=EPODOC&CC=CN&NR=105349530A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HU LIN</creatorcontrib><creatorcontrib>JIN RONGYU</creatorcontrib><creatorcontrib>FAN YE</creatorcontrib><creatorcontrib>GAO QIUPING</creatorcontrib><creatorcontrib>WANG DAISANG</creatorcontrib><creatorcontrib>ZHANG JIANXUN</creatorcontrib><creatorcontrib>QI CHEN</creatorcontrib><creatorcontrib>WANG YU</creatorcontrib><creatorcontrib>ZHAO XIN</creatorcontrib><creatorcontrib>SUN GANG</creatorcontrib><creatorcontrib>PENG SHANMING</creatorcontrib><creatorcontrib>YU ZHUJUN</creatorcontrib><creatorcontrib>YU JUNWEI</creatorcontrib><creatorcontrib>YOU QIMIN</creatorcontrib><creatorcontrib>FU JING</creatorcontrib><creatorcontrib>ZHOU YANQIONG</creatorcontrib><creatorcontrib>WANG SHA</creatorcontrib><creatorcontrib>ZHU LUOLUO</creatorcontrib><creatorcontrib>WANG CHEN</creatorcontrib><creatorcontrib>FAN XINLONG</creatorcontrib><creatorcontrib>WU ZHIHONG</creatorcontrib><creatorcontrib>ZHANG QIANGZHONG</creatorcontrib><creatorcontrib>HUANG LONGMEI</creatorcontrib><creatorcontrib>HE JUNLI</creatorcontrib><title>New type nucleic acid detection method and detector tube</title><description>The invention discloses a new type nucleic acid detection method. Lysate, cleanout fluid and reaction liquid in a detector tube are isolated by arranging a plurality of separating layers in the detector tube, meanwhile, through the control on an external heat source, the separating layers are respectively melted in different detection stages so as to control time sequence in all detection stages, a mixing device which is controlled by an external magnetic body to move and can be magnetized is used in the lysate, mixes samples added into the detector tube, and nucleic acid extraction and purification of the samples are realized, then nano magnetic beads in the detector tube are driven by the external magnetic body to pass the cleanout fluid and then enter into the reaction liquid, nucleic acid carried by the nano magnetic beads is purified in the cleanout fluid and then is eluted in the reaction liquid, an amplified reaction of the nucleic acid in the reaction liquid occurs, finally, an external device realizes the detection on the sample nucleic acid by the optical detection, so that a plurality of steps of performing extraction, purification, cleaning, elution and amplified reaction on the nucleic acid in one detector tube are realized.</description><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2016</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLDwSy1XKKksSFXIK03OSc1MVkhMzkxRSEktSU0uyczPU8hNLcnIT1FIzIMJ5hcplJQmpfIwsKYl5hSn8kJpbgZFN9cQZw_d1IL8-NTigsTk1LzUknhnP0MDU2MTS1NjA0djYtQAAOI0Lfg</recordid><startdate>20160224</startdate><enddate>20160224</enddate><creator>HU LIN</creator><creator>JIN RONGYU</creator><creator>FAN YE</creator><creator>GAO QIUPING</creator><creator>WANG DAISANG</creator><creator>ZHANG JIANXUN</creator><creator>QI CHEN</creator><creator>WANG YU</creator><creator>ZHAO XIN</creator><creator>SUN GANG</creator><creator>PENG SHANMING</creator><creator>YU ZHUJUN</creator><creator>YU JUNWEI</creator><creator>YOU QIMIN</creator><creator>FU JING</creator><creator>ZHOU YANQIONG</creator><creator>WANG SHA</creator><creator>ZHU LUOLUO</creator><creator>WANG CHEN</creator><creator>FAN XINLONG</creator><creator>WU ZHIHONG</creator><creator>ZHANG QIANGZHONG</creator><creator>HUANG LONGMEI</creator><creator>HE JUNLI</creator><scope>EVB</scope></search><sort><creationdate>20160224</creationdate><title>New type nucleic acid detection method and detector tube</title><author>HU LIN ; JIN RONGYU ; FAN YE ; GAO QIUPING ; WANG DAISANG ; ZHANG JIANXUN ; QI CHEN ; WANG YU ; ZHAO XIN ; SUN GANG ; PENG SHANMING ; YU ZHUJUN ; YU JUNWEI ; YOU QIMIN ; FU JING ; ZHOU YANQIONG ; WANG SHA ; ZHU LUOLUO ; WANG CHEN ; FAN XINLONG ; WU ZHIHONG ; ZHANG QIANGZHONG ; HUANG LONGMEI ; HE JUNLI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN105349530A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2016</creationdate><topic>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>HU LIN</creatorcontrib><creatorcontrib>JIN RONGYU</creatorcontrib><creatorcontrib>FAN YE</creatorcontrib><creatorcontrib>GAO QIUPING</creatorcontrib><creatorcontrib>WANG DAISANG</creatorcontrib><creatorcontrib>ZHANG JIANXUN</creatorcontrib><creatorcontrib>QI CHEN</creatorcontrib><creatorcontrib>WANG YU</creatorcontrib><creatorcontrib>ZHAO XIN</creatorcontrib><creatorcontrib>SUN GANG</creatorcontrib><creatorcontrib>PENG SHANMING</creatorcontrib><creatorcontrib>YU ZHUJUN</creatorcontrib><creatorcontrib>YU JUNWEI</creatorcontrib><creatorcontrib>YOU QIMIN</creatorcontrib><creatorcontrib>FU JING</creatorcontrib><creatorcontrib>ZHOU YANQIONG</creatorcontrib><creatorcontrib>WANG SHA</creatorcontrib><creatorcontrib>ZHU LUOLUO</creatorcontrib><creatorcontrib>WANG CHEN</creatorcontrib><creatorcontrib>FAN XINLONG</creatorcontrib><creatorcontrib>WU ZHIHONG</creatorcontrib><creatorcontrib>ZHANG QIANGZHONG</creatorcontrib><creatorcontrib>HUANG LONGMEI</creatorcontrib><creatorcontrib>HE JUNLI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HU LIN</au><au>JIN RONGYU</au><au>FAN YE</au><au>GAO QIUPING</au><au>WANG DAISANG</au><au>ZHANG JIANXUN</au><au>QI CHEN</au><au>WANG YU</au><au>ZHAO XIN</au><au>SUN GANG</au><au>PENG SHANMING</au><au>YU ZHUJUN</au><au>YU JUNWEI</au><au>YOU QIMIN</au><au>FU JING</au><au>ZHOU YANQIONG</au><au>WANG SHA</au><au>ZHU LUOLUO</au><au>WANG CHEN</au><au>FAN XINLONG</au><au>WU ZHIHONG</au><au>ZHANG QIANGZHONG</au><au>HUANG LONGMEI</au><au>HE JUNLI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>New type nucleic acid detection method and detector tube</title><date>2016-02-24</date><risdate>2016</risdate><abstract>The invention discloses a new type nucleic acid detection method. Lysate, cleanout fluid and reaction liquid in a detector tube are isolated by arranging a plurality of separating layers in the detector tube, meanwhile, through the control on an external heat source, the separating layers are respectively melted in different detection stages so as to control time sequence in all detection stages, a mixing device which is controlled by an external magnetic body to move and can be magnetized is used in the lysate, mixes samples added into the detector tube, and nucleic acid extraction and purification of the samples are realized, then nano magnetic beads in the detector tube are driven by the external magnetic body to pass the cleanout fluid and then enter into the reaction liquid, nucleic acid carried by the nano magnetic beads is purified in the cleanout fluid and then is eluted in the reaction liquid, an amplified reaction of the nucleic acid in the reaction liquid occurs, finally, an external device realizes the detection on the sample nucleic acid by the optical detection, so that a plurality of steps of performing extraction, purification, cleaning, elution and amplified reaction on the nucleic acid in one detector tube are realized.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | New type nucleic acid detection method and detector tube |
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