Preparation method of human milk fat substitute, human milk fat substitute prepared by method, and use of milk fat substitute

The invention relates to a preparation method of a human milk fat substitute, the human milk fat substitute prepared by the method, and use of the milk fat substitute. Specifically, edible fat is used as a raw material, and a random ester exchange reaction, a physical mixed reaction and a directed ester exchange reaction are performed, so that the human milk fat substitute is prepared. In the human milk fat substitute disclosed by the invention, compared with the total weight of fatty acid residues at an Sn-2 site of triglyceride of the human milk fat substitute, the content of palmitic acid residues at the Sn-2 site is 20wt%-60wt%, and the content of linoleic acid at the Sn-2 site is 3wt%-15wt%; compared with the total weight of the fatty acid residues in the triglyceride of the human milk fat substitute, the total content of the linolenic acid is 1wt%-5wt%. According to the method disclosed by the invention, the edible fat with broad sources is used as the raw material, and the technology is simple, so that the mass production of the human milk fat substitute of which the structure is similar to that of breast milk triglyceride in industry at a low cost can be realized. The human milk fat substitute prepared by the method disclosed by the invention is easy to absorb by babies, and promotion of the growth and the development of the babies is facilitated.