Liquid chromatography for separating and determining pomalidomide intermediate related substance

The invention discloses a liquid chromatography for separating and determining a pomalidomide intermediate related substance. The method comprises the following steps: 1) a tested object solution and a reference solution are prepared; 2) the tested object solution and the reference solution are detected through high-performance liquid chromatographic determination; wherein chromatogram condition is characterized in that octadecylsilane chemically bonded silica (250*4.0 mm, 5 [mu]m) is taken as a filler, an acetonitrile/phosphoric acid buffer (a pH value is 2.6) with a ratio of 10:90 is taken as a mobile phase A, the acetonitrile/phosphoric acid buffer (the pH value is 2.6) with the ratio of 90:10 is taken as the mobile phase B, gradient elution is carried out, a column temperature is 25-40 DEG C, a flow velocity is 0.8-1.2 ml/min, and a detection wavelength is 254 nm.