Prawn covert mortality nodavirus fluorescent quantitative RT-PCR detection method

The invention relates to a prawn covert mortality nodavirus (CMNV) fluorescent quantitative PCR (RT-PCR) detection method. The method includes the steps that a primer and a Taqman probe are designed according to a RNA polymerase gene conserved gene segment on which RNA of the CMNV depends, an RNA sample is prepared, and fluorescent quantitative RT-PCR is carried out according to preset reaction conditions. By means of standard curve manufacturing and specificity verification of the detection method, results show that absolute quantification of the CMNV can be achieved through the method. Meanwhile, the CMNV can be accurately identified from the white spot syndrome virus, the baculovirus, the taura syndrome virus, the hepatopancreatic parvovirus, the infectious hypodermal and hematopoietic necrosis virus and the infectious myonecrosis virus through the method, and high specificity is represented. The primer, the probe and the detection method have high popularization value in enhancement of monitoring of the CMNV in water, bait and offspring seeds and prevention of large-scale epidemic outbreak of the CMNV.