Real-time fluorescence PCR (polymerase chain reaction) primer and probe combination for simultaneously detecting 4 pathogenic bacteria causing Bull's-eye rot of apple

Real-time fluorescence PCR (polymerase chain reaction) primer and probe combination for simultaneously detecting 4 pathogenic bacteria causing Bull's-eye rot of apple

The invention provides a real-time fluorescence PCR (polymerase chain reaction) primer and probe combination for simultaneously detecting 4 pathogenic bacteria causing Bull's-eye rot of apple. The sequences of the primer and probe combination are represented as SEQ ID No.1-6. Furthermore, a rea...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: WANG WEIFANG, LIN HUIJIAO, HU XUENAN, HE RIRONG
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROCESSES USING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention provides a real-time fluorescence PCR (polymerase chain reaction) primer and probe combination for simultaneously detecting 4 pathogenic bacteria causing Bull's-eye rot of apple. The sequences of the primer and probe combination are represented as SEQ ID No.1-6. Furthermore, a real-time fluorescence PCR method for simultaneously detecting the 4 pathogenic bacteria causing Bull's-eye rot of apple is established according to the primer and probe combination. With the adoption of the method, the detection of the 4 pathogenic bacteria causing Bull's-eye rot of apple can be realized. According to the real-time fluorescence PCR primer and probe combination and the detection method, good specificity, high flux and rapidness are realized, the detection efficiency is improved, and the more effective method is provided for detection of the 4 pathogenic bacteria causing Bull's-eye rot of apple.