Disinfection and inoculation method of microphyte seeds and pollen

The invention discloses a disinfection and inoculation method of microphyte seeds and pollen. A special disinfection and inoculation technology for small seeds and plant pollen in a tissue culture process is designed by utilizing seeds and plant pollen of microphytes including orchids as materials in combination of a disinfection method of the tissue culture technology and tools such as an injector and the like; according to the technology, the problems that effective disinfection cannot be realized and a disinfectant cannot be completely removed due to too small orchid seeds and most plant pollen are solved, and the contamination rate in the tissue culture can be effectively reduced to be lower than 5%. Besides, with the adoption of the technology, disinfection and inoculation speeds can be greatly increased, meanwhile, due to the fact that a low-concentration sodium hypochlorite solution can effectively destroy seed coats of the microphyte seeds and cell walls of the pollen and cannot damage seed embryos and pollen cells, accordingly, the microphyte seeds of the plants such as the orchids and the like or the pollen cells of the plants can absorb nutrition of a culture medium more easily, and the seeds germinate 3-5 days in advance.