Hnl mutants at low ph

The present invention relates to newly identified mutants of (R)-selective hydroxynitrile lyase enzyme (R-HNL), in particular variants of Pa HNL (hydroxynitrile lyase isoenzyme 5 of Prunus amygdalus) which are stable at very low pH, i.e. at pH around 2.5 and lower. In particular, the present inventi...

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Bibliographische Detailangaben
Hauptverfasser:	WAECHTER RALPH, PETSCHACHER BARBARA, GLIEDER ANTON, WEINHANDL KATRIN, ACHKAR JIHANE, CALLANT DOMINIQUE MONIQUE CHARLES, WIERTZ ROEL WIM
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to newly identified mutants of (R)-selective hydroxynitrile lyase enzyme (R-HNL), in particular variants of Pa HNL (hydroxynitrile lyase isoenzyme 5 of Prunus amygdalus) which are stable at very low pH, i.e. at pH around 2.5 and lower. In particular, the present invention provides nucleic acids encoding said mutant enzymes, recombinant microorganisms and their use for industrial applications.