Fluorescent PCR detecting kit for V600E mutation of BRAF gene

The invention provides a fluorescent PCR detecting kit for V600E mutation of a BRAF gene. The detecting kit comprises a V600E mutation detection reaction solution, wherein the V600E mutation detection reaction solution comprises a 10*PCR buffering solution, deoxyribonucleoside triphosphate, an upstream primer, a downstream primer and a probe, wherein the upstream primmer and the downstream primer are used for amplifying target polynucleotide, and the probe is used for detecting the target polynucleotide. The fluorescent PCR detecting kit for V600E mutation of the BRAF gene is based on a real-time fluorescent quantitative PCR technology and an ARMS-PCR technology, has favorable specificity and is high in operation speed, simple and convenient in method and high in detection precision; and meanwhile an interior label additionally arranged in a reaction system can be used for effectively avoiding that the detected result is false negative.