Isothermal amplification detection kit for pork derived component nucleic acid and detection method

The invention discloses an isothermal amplification detection kit for pork derived component nucleic acid and a detection method. The kit contains an isothermal amplification reaction liquid, positive control and negative control. The detection kit provided by the invention has high specificity and...

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Bibliographische Detailangaben
Hauptverfasser:	SHI YA, XU CHANGPING, HU ZONGYUE, LU YIYU, XU ZHOUHENG, FENG YAN
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses an isothermal amplification detection kit for pork derived component nucleic acid and a detection method. The kit contains an isothermal amplification reaction liquid, positive control and negative control. The detection kit provided by the invention has high specificity and high sensitivity. Nucleic acid amplification reaction is constantly at a same temperature, isothermal amplification only needs 30min, nucleic acid test strip detection result can be obtained in 1-2min, the whole process from receiving a sample till getting the result needs only about 40min, and the whole reaction process only needs a constant temperature apparatus. Thus, the kit and the method are especially for field fast detection of pork derived component nucleic acid.