Method and kit for mutation detection of mitochondrion tRNA<Leu(UUR)>3253T>C

The invention discloses a method and a kit for mutation detection of a mitochondrion tRNA3253T>C. The method includes DNA (deoxyribonucleic acid) extraction, specific primer design, specific band PCR (polymerase chain reaction) amplification, specific restriction enzyme BstEII digestion, agarose...

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Bibliographische Detailangaben
Hauptverfasser:	ZHENG BINJIAO, XUE LING, TANG XIAOWEN, GUAN MINXIN, LIN ZHI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a method and a kit for mutation detection of a mitochondrion tRNA3253T>C. The method includes DNA (deoxyribonucleic acid) extraction, specific primer design, specific band PCR (polymerase chain reaction) amplification, specific restriction enzyme BstEII digestion, agarose gel electrophoresis and the like. Results can be obtained within a few hours, and one enzyme is only capable of identifying one specific deoxyribonucleotide sequence, so that accuracy of the results is realized. The invention further provides a kit for mutation detection of the mitochondrion tRNA3253T>C related to primary hypertension. The method and the kit for mutation detection of the mitochondrion tRNA3253T>C have the advantages of low requirements on instruments for detection, low cost of required reagents, simplicity and easiness in operation, stability of the method, high specificity and the like.