Grass carp polymeric immunoglobulin receptor (pIgR) gene full-length cDNA and cloning method and application thereof

Grass carp polymeric immunoglobulin receptor (pIgR) gene full-length cDNA and cloning method and application thereof

The invention relates to grass carp polymeric immunoglobulin receptor (pIgR) gene full-length cDNA and a cloning method and application thereof. The grass carp polymeric immunoglobulin receptor (pIgR) gene full-length cDNA is characterized in that the accession number of a Genbank is KP768418, the G...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: TIAN GONGTAI, DU XINGHUA, LIU FENG, ZHANG MINGLEI, LIU FEI, XU GUOJING, WANG CHAO, DONG GUANCANG, MENG QINGLEI
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention relates to grass carp polymeric immunoglobulin receptor (pIgR) gene full-length cDNA and a cloning method and application thereof. The grass carp polymeric immunoglobulin receptor (pIgR) gene full-length cDNA is characterized in that the accession number of a Genbank is KP768418, the Genbank comprises a 1011bp open reading frame, 336 amino acids are coded, a main binding locus CWDC exists, unique and conservative binding loci KxWC and DxGxYxC and conservative cysteine residues exist in a functional zone, 5'UTR is 68bp, and 3'UTR is 561bp, wherein a termination codon and a polyadenosine tailing signal are contained. According to the grass carp polymeric immunoglobulin receptor (pIgR) gene full-length cDNA and the cloning method and application thereof, cloning of pIgR gene cDNA is convenient, biological materials are easy to obtain, prevention of fish diseases and evaluation of vaccine use effect can be conducted, and important theoretical and practical significance to aquaculture is achieved.