Tissue culture method of lepidium sativum

The invention discloses a tissue culture method of lepidium sativum. The method comprises the following steps: first acquiring a sterile germinated seedling, then cutting the stem of the sterile germinated seedling of the lepidium sativum for standby application on a sterile operation table, taking hypocotyls of a well cut explant, inoculating into a callus tissue induction culture medium to acquire callus, inoculating the well-growing callus to an adventitious bud induction culture medium cured by agar to culture and obtain cespitose adventitious buds, transferring the adventitious buds into a rooting culture medium, and carrying out the rooting culture to obtain a rooted small plant. By adopting the method, the rapid propagation system of cluster buds of the lepidium sativum is optimized, and the production can be further enlarged.