Application of beta-glucosidase and method for preparing baicalein through beta-glucosidase

The invention relates to the technical field of biology, in particular to an application of beta-glucosidase and a method for preparing baicalein through the beta-glucosidase. The amino acid sequence of the beta-glucosidase is shown as SEQ ID No.1. The invention further provides a baicalin enzyme hydrolysis method. The heat-resisting and sugar-resisting beta-glucosidase is immobilized to hydrolyze the baicalin in a buffer solution, and the baicalein is obtained. The beta-glucosidase has high product tolerance, the baicalin can be completely hydrolyzed without being in a double-phase system, and it is avoided that due to organic solvents in the double-phase system, the activity of the glucosidase is reduced. The immobilized glucosidase can be repeatedly used. By means of the application and the method, the high-purity baicalein is obtained, and the purity is higher than 95%. According to the method, after the baicalein is extracted through ethyl acetate, recrystallization can be carried out through organic solvents, the high-purity baicalein can be obtained, and the purity is higher than 98%.