Glycine max transformation event SHZD32-01 and detection method thereof
The invention discloses a glycine max plant containing a glycine max transformation event SHZD32-01 and a unique DNA molecule in the glycine max transformation event SHZD32-01 and further provides a detection method for the unique DNA molecule. The glycine max transformation event SHZD32-01 contains...
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creator | CAO YUEPING |
description | The invention discloses a glycine max plant containing a glycine max transformation event SHZD32-01 and a unique DNA molecule in the glycine max transformation event SHZD32-01 and further provides a detection method for the unique DNA molecule. The glycine max transformation event SHZD32-01 contains a molecule comprising at least one of the following nucleic acid sequences: SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, and SEQ ID NO.9 as well as complete complementary sequences; the detection method comprises the following steps: 1), mixing samples comprising DNA and primers; 2), carrying out nucleic acid amplification reaction so as to generate an amplification product; 3), detecting the amplification product, wherein the amplification product comprise SEQ ID NO.1 or SEQ ID NO.2. The glycine max plant containing the glycine max transformation event SHZD32-01 shows great glyphosate tolerance, and is beneficial to control over weeds; and the DNA detection of the glycine max transformation event SHZD32-01 has a high value for identifying the glycine max transformation event SHZD32-01 in the sample and breeding of the glycine max containing the DNA. |
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The glycine max transformation event SHZD32-01 contains a molecule comprising at least one of the following nucleic acid sequences: SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, and SEQ ID NO.9 as well as complete complementary sequences; the detection method comprises the following steps: 1), mixing samples comprising DNA and primers; 2), carrying out nucleic acid amplification reaction so as to generate an amplification product; 3), detecting the amplification product, wherein the amplification product comprise SEQ ID NO.1 or SEQ ID NO.2. The glycine max plant containing the glycine max transformation event SHZD32-01 shows great glyphosate tolerance, and is beneficial to control over weeds; and the DNA detection of the glycine max transformation event SHZD32-01 has a high value for identifying the glycine max transformation event SHZD32-01 in the sample and breeding of the glycine max containing the DNA.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160106&DB=EPODOC&CC=CN&NR=105219768A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20160106&DB=EPODOC&CC=CN&NR=105219768A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CAO YUEPING</creatorcontrib><title>Glycine max transformation event SHZD32-01 and detection method thereof</title><description>The invention discloses a glycine max plant containing a glycine max transformation event SHZD32-01 and a unique DNA molecule in the glycine max transformation event SHZD32-01 and further provides a detection method for the unique DNA molecule. The glycine max transformation event SHZD32-01 contains a molecule comprising at least one of the following nucleic acid sequences: SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, and SEQ ID NO.9 as well as complete complementary sequences; the detection method comprises the following steps: 1), mixing samples comprising DNA and primers; 2), carrying out nucleic acid amplification reaction so as to generate an amplification product; 3), detecting the amplification product, wherein the amplification product comprise SEQ ID NO.1 or SEQ ID NO.2. 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The glycine max transformation event SHZD32-01 contains a molecule comprising at least one of the following nucleic acid sequences: SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, and SEQ ID NO.9 as well as complete complementary sequences; the detection method comprises the following steps: 1), mixing samples comprising DNA and primers; 2), carrying out nucleic acid amplification reaction so as to generate an amplification product; 3), detecting the amplification product, wherein the amplification product comprise SEQ ID NO.1 or SEQ ID NO.2. The glycine max plant containing the glycine max transformation event SHZD32-01 shows great glyphosate tolerance, and is beneficial to control over weeds; and the DNA detection of the glycine max transformation event SHZD32-01 has a high value for identifying the glycine max transformation event SHZD32-01 in the sample and breeding of the glycine max containing the DNA.</abstract><oa>free_for_read</oa></addata></record> |
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subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
title | Glycine max transformation event SHZD32-01 and detection method thereof |
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