Glycine max transformation event SHZD32-01 and detection method thereof

The invention discloses a glycine max plant containing a glycine max transformation event SHZD32-01 and a unique DNA molecule in the glycine max transformation event SHZD32-01 and further provides a detection method for the unique DNA molecule. The glycine max transformation event SHZD32-01 contains a molecule comprising at least one of the following nucleic acid sequences: SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, and SEQ ID NO.9 as well as complete complementary sequences; the detection method comprises the following steps: 1), mixing samples comprising DNA and primers; 2), carrying out nucleic acid amplification reaction so as to generate an amplification product; 3), detecting the amplification product, wherein the amplification product comprise SEQ ID NO.1 or SEQ ID NO.2. The glycine max plant containing the glycine max transformation event SHZD32-01 shows great glyphosate tolerance, and is beneficial to control over weeds; and the DNA detection of the glycine max transformation event SHZD32-01 has a high value for identifying the glycine max transformation event SHZD32-01 in the sample and breeding of the glycine max containing the DNA.