Preparation method of actinomycetes fibrinolytic enzyme

The invention relates to a preparation method of an actinomycetes fibrinolytic enzyme. Specifically, the method comprises the following steps: (1) adding ammonium sulfate powder to a fermentation supernatant containing actinomycetes fibrinolytic enzyme until a saturation degree is 25-35%W/V, standing by for a whole night at 4 DEG C and centrifuging so as to obtain a supernatant, adding ammonium sulfate powder to the supernatant once again until a saturation degree is 55-65%W/V, standing by for a whole night at 4 DEG C, centrifuging so as to obtain a precipitate, and dissolving the precipitate by virtue of a PB buffer solution so as to obtain crude enzyme liquid; and (2) centrifuging the crude enzyme liquid obtained from the step (1) at low temperature so as to remove impurities; purifying an obtained supernatant subsequently through Octyl-Sepharose FF hydrophobic interaction chromatography and Phenyl-Sepharose HP hydrophobic interaction chromatography so as to obtain a chromatographically pure enzyme; dialyzing and desalting the enzyme, and freeze-drying the enzyme so as to obtain fibrinolytic enzyme freeze-dried powder which can be preserved for a long time at minus 20 DEG C. The separation and purification method of the actinomycetes fibrinolytic enzyme disclosed by the invention has the advantages of simple operation steps and high recovery rate.