Characteristic protein for identifying Chinese date honey flower source

The invention relates to a Chinese date honey characteristic protein and isolation and purification thereof; firstly, eight single-flower honey is subjected to SDS-PAGE, a result finds out that the Chinese date honey has one protein spectral band at about 17 ku while protein electrophoresis spectrog...

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Bibliographische Detailangaben
Hauptverfasser: CHEN NI, ZHOU HOUBAO, CAO WEI, GAO HUI
Format: Patent
Sprache:eng
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Zusammenfassung:The invention relates to a Chinese date honey characteristic protein and isolation and purification thereof; firstly, eight single-flower honey is subjected to SDS-PAGE, a result finds out that the Chinese date honey has one protein spectral band at about 17 ku while protein electrophoresis spectrograms of other honeys do not contain the protein spectral band at about 17 ku; in addition, the molecular weight of the protein has quite large difference from the molecular weights of other protein spectral bands, so as to facilitate separation. Therefore, the protein can be used as the characteristic protein of the Chinese date honey different from other honeys. For making the molecular weight and structure information of the protein clear, an LC-Chip/ESI-QTOF-MS method is used for identification. The operation is simple, the separation effect is good, the feasibility is high, and the new method is provided for true and false identification of the Chinese date honey.