Separation method of soluble boron in plants

The invention discloses a separation method of soluble boron in plants. The separation method comprises the following steps: (1) washing, drying, crushing and sieving plant samples; (2) weighing a certain amount of powder sample and putting the powder sample into a needle cylinder with a sieve plate and an end cap; adding water to immerse the sample and ultrasonically vibrating; (3) taking off the end cap of the needle cylinder of an injection syringe and putting the needle cylinder of the injection syringe into a screw-cap centrifugal pipe; centrifuging, separating and collecting filtrates; and (4) repeating the step (2) of adding the water and vibrating, and the step (3), totally separating for 5-10 times, and combining all the filtrates so as to completely separate the soluble boron in the plant samples. The invention establishes the separation method of the soluble boron in the plants; the method can be used for separating the soluble boron in plant sample fluid from non-soluble boron in tissue residues; the method has the characteristics of simplicity and reliability, and wide application prospect, and provides new technical supports for researches in the fields including plant boron nutrition, physiology and biochemistry of the boron, biogeochemical cycles of the boron and the like.