Adenovirus titer detection method

The invention discloses an adenovirus titer detection method, that is, a TCID50 method. The method comprises the following steps: paving HEK293 cells in the logarithmic growth stage in a cell culture plate according to a cell density of 2*10 /0.1mL/hole, and culturing the cells in a culture box to m...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	DAI DONGSHENG, WANG FEI, YANG RENJIA, JIAO JIN'AN, ZHOU PENG, WANG XINGXING, CHEN JING
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention discloses an adenovirus titer detection method, that is, a TCID50 method. The method comprises the following steps: paving HEK293 cells in the logarithmic growth stage in a cell culture plate according to a cell density of 2*10 /0.1mL/hole, and culturing the cells in a culture box to make the HEK293 cells be attached to the bottom of holes; and carrying out gradient dilution on detected adenoviruses, inoculating the diluted adenoviruses into the HEK293 cells, incubating for 60min, changing to a maintenance solution, placing the culture plate in the culture box, culturing, recording the cytopathic hole number 10d later, and calculating the TCID50 of the adenoviruses according to a formula. The adenovirus titer detection method TCID50 method is more accurate and stable than a PFU method.