Method for expressing collagen by means of dual-oleosin fusion technology

The invention discloses an oil body containing dual-oleosin and collagen oligopeptide fusion protein. Dual-oleosin of arabidopsis thaliana is fused with recombinant collagen oligopeptides by means of the recombinant DNA technology, a fusion gene expression plasmid expression vector pKO-OL-OL-Col is...

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Hauptverfasser: WEI JIAN, GAO LIHONG, GAI YUHONG, DING YI'NAN, YANG YANPING, ZHU NI'NA, LU CHENGWEN, SONG KAI, MO JIN'GANG, ZHANG LIJIAO
Format: Patent
Sprache:eng
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Zusammenfassung:The invention discloses an oil body containing dual-oleosin and collagen oligopeptide fusion protein. Dual-oleosin of arabidopsis thaliana is fused with recombinant collagen oligopeptides by means of the recombinant DNA technology, a fusion gene expression plasmid expression vector pKO-OL-OL-Col is established and transformed into oil crops, fusion protein is expressed, and the oil body containing the dual-oleosin and the collagen oligopeptide fusion protein is obtained. Operation is simple, the cost is low, the expression quantity is high, the oil body containing the dual-oleosin and the collagen oligopeptide fusion protein has higher collagen activity and can be directly applied to production of cosmetics, the production cost is reduced, the processes of collagen purification and emulsifier or protective agent mixing are omitted, and the dual-oleosin and the fusion protein are integrated to well protect the recombinant collagen oligopeptides.