Method for mixed culture and re-separation of riemerella anatipestifer and escherichia coli

The invention discloses a method for mixed culture and re-separation of riemerella anatipestifer and escherichia coli. The method for mixed culture and re-separation of riemerella anatipestifer and escherichia coli comprises the following steps of (1) performing strain recovery; (2) screening riemerella anatipestifer and escherichia coli with antibiotics sensitivity differences; (3) inoculating the riemerella anatipestifer and the escherichia coli into a culture medium for mixed culture at a certain time interval or different diluting concentration; (4) measuring the minimum inhibitory concentration of antibiotics in the second step on the screened riemerella anatipestifer and escherichia coli; (5) preparing an antibiotic plate culture medium; (6) separating mixed culture bacterium liquid. The method for mixed culture and re-separation of riemerella anatipestifer and escherichia coli has the advantages that two kinds of bacteria are used outside duck poultry bodies and can jointly grow for more than 20 generations; conditions are provided for studying drug resistance transfer and relevant mechanisms of the two kinds of bacteria under the symbiosis condition; important significance is realized on disease prevention and control of duck breeding industry.