Peptide vaccine for animals and preparing thereof

The invention provides synthetic peptide vaccine for a foot-and-mouth disease, and particularly relates to polypeptide for the synthetic peptide vaccine for the foot-and-mouth disease, the vaccine with the polypeptide, a preparing method of the polypeptide and a preparing method of the vaccine. The polypeptide has the amino acid sequences shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6. According to the polypeptide, the vaccine and the preparing methods, by determining the sequences of recent foot-and-mouth disease prevalent strains in China, the variation conditions of main antigen sites of the strains are researched in cooperation with the sequences of foot-and-mouth disease vaccine strains; meanwhile, analytical predication of the antigen sites is carried out in cooperation with computer assistance, and possible antigen site peptide fragments are chemically synthesized. Candidate polypeptide antigens are screened through a large quantity of animal experiments, the foot-and-mouth disease virus antigen sites are optimized according to the screening result, T-cell epitope and B-cell epitope are effectively combined, and the immune effect of the polypeptide antigens is improved. The synthetic peptide vaccine for the foot-and-mouth disease can effectively respond to foot-and-mouth disease virus antigen variations, is good in biological safety and beneficial for large-scale synthesis and has good application prospects.