Cultivation method of round radish cytoplasmic male novel sterile line

The invention relates to a cultivation method of a round radish cytoplasmic male novel sterile line. The method comprises the steps of selecting radish seeds of a female parent sterile line 262A and radish seeds of a male parent selfing line XYH-3-1-5 for conducting corresponding-period sowing; after seedlings of the radish seeds of a female parent and the radish seeds of a male parent emerge and one week passes, conducting thinning; after the age of the radish seedlings reaches 50-60 days, planting the seedlings to a separation net room; after radish plants survive, conducting weeding and ridging in combination with soil loosening; before the radish plants flower, adopting bees for pollination; after flowers of the radish plants fade, withdrawing the bees, and uprooting the male parent selfing line XYH-3-1-5; after radish pods are ripe, conducting harvesting, and conducting after-ripening and threshing in the net room; obtaining the round radish cytoplasmic male novel sterile line (262A * XYH-3-1-5)A, namely harvested radish seeds. The novel sterile line cultivated out through the cultivation method has the advantages of being great in stress resistance, vigorous in plant growth and the like.