Kit for quickly detecting rare deletional thalassemia based on hydrolysis probe method

The invention discloses a kit for quickly detecting rare deletional thalassemia based on a hydrolysis probe method. The kit comprises a pair of primers amplifying characteristic sequences of -- alleles in alpha-globin gene clusters, a pair of primers amplifying HPFH-SEA alleles in beta-globin gene c...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	PANG WANRONG, SUN LEI, LONG JU, YE XUEHE
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention discloses a kit for quickly detecting rare deletional thalassemia based on a hydrolysis probe method. The kit comprises a pair of primers amplifying characteristic sequences of -- alleles in alpha-globin gene clusters, a pair of primers amplifying HPFH-SEA alleles in beta-globin gene clusters, a pair of primers amplifying DBT alleles in beta-globin gene clusters, a pair of primers amplifying alleles in alpha 1 fragments of alpha globin gene clusters, a probe for specific detection of -- genotype amplification products, a probe for specific detection of HPFH-SEA genotype amplification products, a probe for specific detection of DBT genotype amplification products and a probe for specific detection of allele amplification products of the alpha1 fragments. The kit has high sensitivity, stability and accuracy and higher specificity to --, HPFH-SEA and DBT.