Kit for quickly detecting sinotype deletional thalassemia based on hydrolysis probe method
The invention discloses a kit for quickly detecting sinotype deletional thalassemia based on a hydrolysis probe method. The kit comprises a pair of primers DBT-F and DBT-R amplifying DBT alleles in beta-globin gene clusters and a fluorescent probe DBT-Prob for specific detection of DBT-F and DBT-R a...
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| creator | PANG WANRONG SUN LEI LONG JU |
| description | The invention discloses a kit for quickly detecting sinotype deletional thalassemia based on a hydrolysis probe method. The kit comprises a pair of primers DBT-F and DBT-R amplifying DBT alleles in beta-globin gene clusters and a fluorescent probe DBT-Prob for specific detection of DBT-F and DBT-R amplification products, wherein the DBT-F is 5'-GGGTGAGGAACAATTGAA AC-3', the DBT-R is 5'-ACCACATCCCTAACACAAC-3', and the DBT-probe is 5'-ROX-ACTATCA CACCCTGCTTACCTCTGATGGA-BHQ-2-3'. The kit can be used for achieving detection of DBT deletional thalassemia alleles through single-tube one-time reaction and has high sensitivity, stability and accuracy and higher specificity. |
| format | Patent |
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The kit comprises a pair of primers DBT-F and DBT-R amplifying DBT alleles in beta-globin gene clusters and a fluorescent probe DBT-Prob for specific detection of DBT-F and DBT-R amplification products, wherein the DBT-F is 5'-GGGTGAGGAACAATTGAA AC-3', the DBT-R is 5'-ACCACATCCCTAACACAAC-3', and the DBT-probe is 5'-ROX-ACTATCA CACCCTGCTTACCTCTGATGGA-BHQ-2-3'. The kit can be used for achieving detection of DBT deletional thalassemia alleles through single-tube one-time reaction and has high sensitivity, stability and accuracy and higher specificity.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2015</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20151216&DB=EPODOC&CC=CN&NR=105154575A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25545,76296</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20151216&DB=EPODOC&CC=CN&NR=105154575A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>PANG WANRONG</creatorcontrib><creatorcontrib>SUN LEI</creatorcontrib><creatorcontrib>LONG JU</creatorcontrib><title>Kit for quickly detecting sinotype deletional thalassemia based on hydrolysis probe method</title><description>The invention discloses a kit for quickly detecting sinotype deletional thalassemia based on a hydrolysis probe method. The kit comprises a pair of primers DBT-F and DBT-R amplifying DBT alleles in beta-globin gene clusters and a fluorescent probe DBT-Prob for specific detection of DBT-F and DBT-R amplification products, wherein the DBT-F is 5'-GGGTGAGGAACAATTGAA AC-3', the DBT-R is 5'-ACCACATCCCTAACACAAC-3', and the DBT-probe is 5'-ROX-ACTATCA CACCCTGCTTACCTCTGATGGA-BHQ-2-3'. 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The kit comprises a pair of primers DBT-F and DBT-R amplifying DBT alleles in beta-globin gene clusters and a fluorescent probe DBT-Prob for specific detection of DBT-F and DBT-R amplification products, wherein the DBT-F is 5'-GGGTGAGGAACAATTGAA AC-3', the DBT-R is 5'-ACCACATCCCTAACACAAC-3', and the DBT-probe is 5'-ROX-ACTATCA CACCCTGCTTACCTCTGATGGA-BHQ-2-3'. The kit can be used for achieving detection of DBT deletional thalassemia alleles through single-tube one-time reaction and has high sensitivity, stability and accuracy and higher specificity.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Kit for quickly detecting sinotype deletional thalassemia based on hydrolysis probe method |
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